Fluorescence:Article Title: In vivo base editing of Chd3 rescues behavioural abnormalities in mice.
Article Snippet: .. Images were acquired using an Olympus VS200 high-throughput fluorescence microscope. .. The primary antibodies used in this study and their dilutions are as follows: rabbit anti-CHD3 (1:1,000 dilution, ab109195, Abcam); rabbit anti-GAPDH (1:3,000 dilution, D264398, Sangon Biotech); rabbit anti-CRISPR–Cas9 (1:500 dilution, ab204448, Abcam); chicken antiNeuN (1:1,000 dilution, ABN91, Millipore); rabbit anti-Flag (1:3,000 dilution, D110005, Sangon Biotech); rabbit anti-TBR1 (1:500 dilution, ab183032, Abcam); rat anti-CTIP2 (1:500 dilution, cab18465, Abcam); mouse anti-SATB2 (1:500 dilution, ab51502, Abcam); rat anti-CUX1 (1:500 dilution, ab307821, Abcam); goat anti-SOX2 (1:500 dilution, AF2018, Biotechne); rat anti-KI67 (1:500 dilution, 14-5698-82, ThermoFisher); goat anti-doublecortin (1:500 dilution, SC-8066, SANTA); rabbit anti-parvalbumin (1:500 dilution, ab181086, Abcam); donkey anti-DAPI (1:1,000 dilution; 28718-90-3, Sigma-Aldrich); donkey anti-rabbit IgG Alexa 488 (1:1,000 dilution; A32790; Thermo Fisher Scientific); donkey anti-rabbit IgG Alexa 555 (1:1,000 dilution; A32794; Thermo Fisher Scientific); donkey anti-mouse IgG Alexa 488 (1:1,000 dilution; A32766; Thermo Fisher Scientific); donkey anti-mouse IgG Alexa 555 (1:1,000 dilution; A32773; Thermo Fisher Scientific); donkey anti-rat IgG Alexa 555 (1:1,000 dilution; A48270; Thermo Fisher Scientific); donkey anti-goat IgG Alexa 555 (1:1,000 dilution; A32816; Thermo Fisher Scientific); and donkey anti-chicken Alexa Fluor 488 (1:1,000; 20166; Biotium).
Article Title: In vivo base editing of Chd3 rescues behavioural abnormalities in mice
Article Snippet: .. Images were acquired using an Olympus VS200 high-throughput fluorescence microscope. .. The primary antibodies used in this study and their dilutions are as follows: rabbit anti-CHD3 (1:1,000 dilution, ab109195, Abcam); rabbit anti-GAPDH (1:3,000 dilution, D264398, Sangon Biotech); rabbit anti-CRISPR–Cas9 (1:500 dilution, ab204448, Abcam); chicken anti-NeuN (1:1,000 dilution, ABN91, Millipore); rabbit anti-Flag (1:3,000 dilution, D110005, Sangon Biotech); rabbit anti-TBR1 (1:500 dilution, ab183032, Abcam); rat anti-CTIP2 (1:500 dilution, cab18465, Abcam); mouse anti-SATB2 (1:500 dilution, ab51502, Abcam); rat anti-CUX1 (1:500 dilution, ab307821, Abcam); goat anti-SOX2 (1:500 dilution, AF2018, Biotechne); rat anti-KI67 (1:500 dilution, 14-5698-82, ThermoFisher); goat anti-doublecortin (1:500 dilution, SC-8066, SANTA); rabbit anti-parvalbumin (1:500 dilution, ab181086, Abcam); donkey anti-DAPI (1:1,000 dilution; 28718-90-3, Sigma-Aldrich); donkey anti-rabbit IgG Alexa 488 (1:1,000 dilution; A32790; Thermo Fisher Scientific); donkey anti-rabbit IgG Alexa 555 (1:1,000 dilution; A32794; Thermo Fisher Scientific); donkey anti-mouse IgG Alexa 488 (1:1,000 dilution; A32766; Thermo Fisher Scientific); donkey anti-mouse IgG Alexa 555 (1:1,000 dilution; A32773; Thermo Fisher Scientific); donkey anti-rat IgG Alexa 555 (1:1,000 dilution; A48270; Thermo Fisher Scientific); donkey anti-goat IgG Alexa 555 (1:1,000 dilution; A32816; Thermo Fisher Scientific); and donkey anti-chicken Alexa Fluor 488 (1:1,000; 20166; Biotium).
Microscopy:Article Title: In vivo base editing of Chd3 rescues behavioural abnormalities in mice.
Article Snippet: .. Images were acquired using an Olympus VS200 high-throughput fluorescence microscope. .. The primary antibodies used in this study and their dilutions are as follows: rabbit anti-CHD3 (1:1,000 dilution, ab109195, Abcam); rabbit anti-GAPDH (1:3,000 dilution, D264398, Sangon Biotech); rabbit anti-CRISPR–Cas9 (1:500 dilution, ab204448, Abcam); chicken antiNeuN (1:1,000 dilution, ABN91, Millipore); rabbit anti-Flag (1:3,000 dilution, D110005, Sangon Biotech); rabbit anti-TBR1 (1:500 dilution, ab183032, Abcam); rat anti-CTIP2 (1:500 dilution, cab18465, Abcam); mouse anti-SATB2 (1:500 dilution, ab51502, Abcam); rat anti-CUX1 (1:500 dilution, ab307821, Abcam); goat anti-SOX2 (1:500 dilution, AF2018, Biotechne); rat anti-KI67 (1:500 dilution, 14-5698-82, ThermoFisher); goat anti-doublecortin (1:500 dilution, SC-8066, SANTA); rabbit anti-parvalbumin (1:500 dilution, ab181086, Abcam); donkey anti-DAPI (1:1,000 dilution; 28718-90-3, Sigma-Aldrich); donkey anti-rabbit IgG Alexa 488 (1:1,000 dilution; A32790; Thermo Fisher Scientific); donkey anti-rabbit IgG Alexa 555 (1:1,000 dilution; A32794; Thermo Fisher Scientific); donkey anti-mouse IgG Alexa 488 (1:1,000 dilution; A32766; Thermo Fisher Scientific); donkey anti-mouse IgG Alexa 555 (1:1,000 dilution; A32773; Thermo Fisher Scientific); donkey anti-rat IgG Alexa 555 (1:1,000 dilution; A48270; Thermo Fisher Scientific); donkey anti-goat IgG Alexa 555 (1:1,000 dilution; A32816; Thermo Fisher Scientific); and donkey anti-chicken Alexa Fluor 488 (1:1,000; 20166; Biotium).
Article Title: In vivo base editing of Chd3 rescues behavioural abnormalities in mice
Article Snippet: .. Images were acquired using an Olympus VS200 high-throughput fluorescence microscope. .. The primary antibodies used in this study and their dilutions are as follows: rabbit anti-CHD3 (1:1,000 dilution, ab109195, Abcam); rabbit anti-GAPDH (1:3,000 dilution, D264398, Sangon Biotech); rabbit anti-CRISPR–Cas9 (1:500 dilution, ab204448, Abcam); chicken anti-NeuN (1:1,000 dilution, ABN91, Millipore); rabbit anti-Flag (1:3,000 dilution, D110005, Sangon Biotech); rabbit anti-TBR1 (1:500 dilution, ab183032, Abcam); rat anti-CTIP2 (1:500 dilution, cab18465, Abcam); mouse anti-SATB2 (1:500 dilution, ab51502, Abcam); rat anti-CUX1 (1:500 dilution, ab307821, Abcam); goat anti-SOX2 (1:500 dilution, AF2018, Biotechne); rat anti-KI67 (1:500 dilution, 14-5698-82, ThermoFisher); goat anti-doublecortin (1:500 dilution, SC-8066, SANTA); rabbit anti-parvalbumin (1:500 dilution, ab181086, Abcam); donkey anti-DAPI (1:1,000 dilution; 28718-90-3, Sigma-Aldrich); donkey anti-rabbit IgG Alexa 488 (1:1,000 dilution; A32790; Thermo Fisher Scientific); donkey anti-rabbit IgG Alexa 555 (1:1,000 dilution; A32794; Thermo Fisher Scientific); donkey anti-mouse IgG Alexa 488 (1:1,000 dilution; A32766; Thermo Fisher Scientific); donkey anti-mouse IgG Alexa 555 (1:1,000 dilution; A32773; Thermo Fisher Scientific); donkey anti-rat IgG Alexa 555 (1:1,000 dilution; A48270; Thermo Fisher Scientific); donkey anti-goat IgG Alexa 555 (1:1,000 dilution; A32816; Thermo Fisher Scientific); and donkey anti-chicken Alexa Fluor 488 (1:1,000; 20166; Biotium).
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